DNA sequencing methods

Citation

(2002), "DNA sequencing methods", Industrial Robot, Vol. 29 No. 1. https://doi.org/10.1108/ir.2002.04929aad.008

DNA sequencing methods

Keyword: Medical

Applicant: The University of Iowa Research Foundation, Iowa City, USAPatent number: US6,258,533Publication date: 10 July 2001Title: Iterative and Regenerative DNA Sequencing Method

This patent describes an iterative and regenerative method for sequencing DNA. The invention provides an alternative approach for sequencing DNA that does not require high resolution separations and that generates signals more amenable to analysis. The method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment and overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artefacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. The methods described can also be easily automated and provide a means for readily analysing DNA from many genetic loci.

The method can be used for identifying a first nucleotide n and a second nucleotide n+x in a double stranded nucleic acid segment. It also provides a means for the rapid sequencing of previously uncharacterised viral, bacterial or protozoan human pathogens, as well as the sequencing of plants and animals of interest to agriculture, conservation, and/or science.