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DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis

Bjorn John Stephen (Department of Biosciences, Manipal University Jaipur, Jaipur, India)
Surabhi Suchanti (Department of Biosciences, Manipal University Jaipur, Jaipur, India)
Devendra Jain (Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technologyu, Udaipur, India)
Harshdeep Dhaliwal (Department of Oral Medicine and Radiology, NIMS Dental College, Jaipur, India)
Vikram Sharma (Department of Oral and Maxillofacial Surgery, NIMS University Dental College, Jaipur, India)
Ramandeep Kaur (Department of Computer Engineering, Poornima Institute of Engineering and Technology, Jaipur, India)
Rajeev Mishra (Department of Life Sciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, India)
Abhijeet Singh (Department of Biosciences, Manipal University Jaipur, Jaipur, India)

Sensor Review

ISSN: 0260-2288

Article publication date: 21 July 2022

Issue publication date: 30 August 2022

84

Abstract

Purpose

Neglected tropical diseases (NTDs) are a set of infectious diseases that primarily affect low-income countries situated near the equator. Effective diagnostic tools hold the key to stemming the spread of these infectious diseases. However, specificity is a major concern associated with current diagnostic protocols. In this regard, electrochemical deoxyribonucleic acid (DNA) biosensors could play a crucial role, as highlighted by renewed interest in their research. The purpose of this study was to highlight the current scenario for the design and development of biosensors for the detection of NTDs related pathogens. This review highlights the different types of factors involved and the modifications used to enhance sensor properties.

Design/methodology/approach

The authors discuss the potential of electrochemical DNA biosensors as efficient, affordable diagnostic tools for the detection of pathogens associated with NTDs by reviewing available literature. This study discusses the biosensor components, mainly the probe selection and type of electrodes used, and their potential to improve the overall design of the biosensor. Further, this study analyses the different nanomaterials used in NTD-based electrochemical DNA biosensors and discusses how their incorporation could improve the overall sensitivity and specificity of the biosensor design. Finally, this study examines the impact such techniques could have in the future on mass screening of NTDs.

Findings

The findings provide an in-depth analysis of electrochemical DNA biosensors for the detection of pathogens associated with NTDs.

Originality/value

This review provides an update on the different types and modifications of DNA biosensors that have been designed for the diagnosis of NTD-related pathogens.

Keywords

Acknowledgements

The authors are very grateful to Manipal University Jaipur for providing necessary facilities.

Author contributions: B.J.S., A.S. and R.M. conceived the idea. B.J.S. contributed to the writing of the manuscript. B.J.S., D.J., S.S. and V.S. prepared figures and tables. B.J.S., H.D., V.S., R.K., A.S. and R.M. reviewed drafts of the paper and approved the final manuscript.

Citation

Stephen, B.J., Suchanti, S., Jain, D., Dhaliwal, H., Sharma, V., Kaur, R., Mishra, R. and Singh, A. (2022), "DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis", Sensor Review, Vol. 42 No. 5, pp. 517-525. https://doi.org/10.1108/SR-11-2021-0429

Publisher

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Emerald Publishing Limited

Copyright © 2022, Emerald Publishing Limited

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